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Sep 15, 2022
Reproductive Biology and Endocrinology
Article Institutional access

Background Circular RNAs (circRNAs) play a vital role in cancer progression. However, there are still numerous circRNAs that have not been functionally explored. Our study aimed to disclose the role of circ CSNK1G1 in triple negative breast cancer (TNBC).
Method The expression of circ CSNK1G1, miR 28 5p and lactate dehydrogenase A (LDHA) mRNA was measured by quantitative real time polymerase chain reaction (qPCR), and the expression of LDHA protein was measured by western blot. Cell proliferation was assessed using MTT assay and colony formation assay. Cell apoptosis was monitored using flow cytometry assay. Cell migration and cell invasion were investigated using transwell assay. Glycolysis progression was assessed according to glucose consumption, lactate production and ATP/ADP ratio. Tumor formation assay in nude mice was conducted to verify the role of circ CSNK1G1 in vivo. The interplays between miR 28 5p and circ CSNK1G1 or LDHA were confirmed by dual luciferase reporter assay.
Result Circ CSNK1G1 was upregulated in TNBC tissues and cells. Circ CSNK1G1 knockdown suppressed cancer cell proliferation, migration, invasion and glycolysis energy metabolism, promoted cell apoptosis in vitro, and blocked tumor growth in vivo. Mechanism analysis showed that circ CSNK1G1 positively regulated LDHA expression by suppressing miR 28 5p. Rescue experiments presented that circ CSNK1G1 played functions by targeting miR 28 5p, and miR 28 5p participated in TNBC progression by degrading LDHA.
Conclusion Circ CSNK1G1 promotes cell proliferation, migration, invasion and glycolysis metabolism during TNBC development by regulating the miR 28 5p/LDHA pathway.

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