Takahisa Ohta

Publications : 76
Aldex : 20
H-index : 19
Citations : 941

Colicin E3 and its immunity genes

Haruhiko Masaki, Takahisa Ohta

Mar 1, 1985 in Journal of Molecular Biology
A DNA segment of plasmid ColE3-CA38 was cloned into pBR328 and its nucleotide sequence was determined. This segment contains the putative promoter-operator region, the structural genes of protein A (gene A) and protein B (gene B) of colicin E3, and a part of gene H. Just behind the promoter region, there is an inverted repeat structure of two `SOS boxes`, the specific binding site of the lexA prot...

biology

Further Studies on rodA Mutant: a Round Morphological Mutant of Escherichia coli K-12 with Wild-Type

Hiroshi Matsuzawa et 5 al.

Jan 1, 1980 in Agricultural and biological chemistry
Escherichia coli rodA mutant AOS151 grows as round cells at 30 and 42°C (H. Matsuzawa, K. Hayakawa, T. Sato, and K. Imahori, J. Bacteriol., 115, 436–442 (1973)). The mutant was found to be resistant to mecillinam at both temperatures. lip+ transductants were prepared by Pl phage transduction via strain AOS151, the cotransduction frequency of round morphology (Rod−) at 42°C with the lip gene being ...


chemistry

Synthesis of starch-graft-polyacrylonitrile hydrolyzate and its characterization

Yasusato Sugahara, Takahisa Ohta

Jan 1, 2001 in Journal of Applied Polymer Science
The graft copolymerization of three vinyl monomer species, acrylonitrile (AN), acrylamide (AAm), and acrylic acid (AA), onto starch was carried out with ceric salt (Ce salt) as an initiator. With 3 mmol/L Ce salt, the monomer activity onto starch decreased in the following order: AN > AAm > AA. Grafting efficiency with AN as the grafting monomer was greater than 90%, but with AA and AAm, it was le...

chemistry

Production of Thermophilic Protease (Aqualysin I) by Fed-Batch Cultures of Recombinant Escherichia coli

Shinji Sakamoto, Takahisa Ohta et 8 al.

Jan 1, 1992
Aqualysin I (AQI) is a subtilisin-type serine protease which is secreted into the culture medium by Thermus aquaticus YT-1, an extreme thermophile. AQI is considerably heat-stable and resistant to denaturing treatments in comparison with other microbial serine proteases. Terada et al. previously reported the production of the enzymatically active AQI by expressing the AQI gene under the control of...

chemistry

Immunity Specificity and Evolution of the Nuclease-Type E Colicins

Haruhiko Masaki et 5 al.

Jan 1, 1992
E-Group colicins are encoded by respective ColE plasmids and kill Escherichia coli cells through binding to a common surface receptor, BtuB, which is also the receptor for phage BF23 and vitamin B12 uptake (Buxton, 1971; DiMasi et al., 1973). Since the host E. coli cells are naturally sensitive to colicins, colicinogenic cells must be protected from the colicin action by means of “immunity”, which...



medicine

Blood concentration of cevimeline hydrochloride hydrate after oral rinsing

Makoto Matsubara et 6 al.

Jan 1, 2013 in Journal of the Japanese Stomatological Society

chemistry

Shikonin induces odontoblastic differentiation of dental pulp stem cells via AKT–mTOR signaling in the presence of CD44

Kunihiro Kajiura et 6 al.

Jan 1, 2020 in bioRxiv
In our previous study, we demonstrated that hyaluronan induces odontoblastic differentiation of dental pulp stem cells via interactions with CD44. However, it remains unclear whether CD44 expression by dental pulp stem cells is required for odontoblastic differentiation. Therefore, we searched for a compound that induces odontoblastic differentiation of dental pulp stem cells, regardless of the ch...

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