Meiko Kimura

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The protective role of conjunctival goblet cell mucin sialylation.

Moe Matsuzawa, Jiro Kitaura et 19 al.

Mar 17, 2023 in Nature communications
Gel-forming mucins secreted by conjunctival goblet cells have been implicated in the clearance of allergens, pathogens, and debris. However, their roles remain incompletely understood. Here we show that human and mouse conjunctival goblet cell mucins have Alcian blue-detectable sialic acids, but not sulfates in the steady state. Interestingly, Balb/c mouse strain lacks this sialylation due to a po...


Influence of Endogenous Proteases and Transglutaminase on Thermal Gelation of Salted Squid Muscle Paste

Shin-Ho Park, Nobuo Seki et 5 al.

Jan 1, 2003 in Journal of Food Science
ABSTRACT: Thermal gelation of salted squid mantle muscle paste was studied in relation to endogenous proteases and transglutaminase. Myosin in the paste was preferentially degraded into 130-kDa and 90-kDa fragments at an optimum temperature of 30 °C. Degradation was inhibited with EDTA or 1,10 phenanthroline, suggesting the presence of metalloproteases. Myosin degradation was markedly reduced abov...


TMAOase, trimethylamine‐N‐oxide demethylase, isa thermostable and active enzyme at 80°C

Meiko Kimura, Nobuo Seki et 2 al.

Jan 1, 2003 in Fisheries Science
: Purified trimethylamine-N-oxide demethylase (TMAOase)from walleye pollack muscle is a thermostable protein that was notinactivated after heating at 80°C for 30 min. The heated enzyme was electrophoresed in the same manner as fornative enzyme. Circular dichroism (CD) spectra for purified enzymechanged reversibly in the temperature range of 10–80°C.As the enzyme was still active at 80°C, the CD sp...


Pollen shells and soluble factors play non-redundant roles in the development of allergic conjunctivitis in mice.

Saaya Fukase, Jiro Kitaura et 17 al.

Aug 21, 2021 in The ocular surface
We aimed to clarify the role of particulate allergen exposure to the conjunctiva in the development of allergic conjunctivitis. We administered ragweed pollen suspension, pollen extract, pollen shell, particulate air pollutants, and their combinations to the mouse conjunctiva five days a week without prior sensitization. Clinical signs were scored. Histological changes, cellular infiltrations, mRN...


Purification and characterization of trimethylamine-N-oxide demethylase from walleye pollack muscle

Meiko Kimura, Ikuo Kimura et 2 al.

Oct 1, 2000 in Fisheries Science
Solubilization of trimethylamine-N-oxide demethylase (TMAOase) from walleye pollack myofibrillar fraction was examined by various treatments using detergents, acetone, and acid solutions. Without detergent treatments, TMAOase could be extracted from the myofibrillar fraction with 1 M NaCl at pH 4.5, and partially purified by diethylaminoethyl (DEAE)-cellulose and gel filtration chromatography. The...

Enzymic and Nonenzymic Cleavage of Trimethylamine-N-oxide in vitro at Subzero Temperatures.

Meiko Kimura, Ikuo Kimura et 2 al.

海産魚類筋肉中kのトリメチルアミンオキシド(TMAO)の分解は凍結貯蔵中にも進行することから,その酵素的および非酵素的分解機構をFe2+と還元剤(アスコルビン酸とCys)の存在下にin vitroで調べた。TMAOaseはスケトウダラ筋肉から調製した。-4℃過冷却未凍結溶液中ではTMAOaseにより酵素濃度依存的にジメチルアミン(DMA)を生成したが,非酵素的分解はほとんど起こらなかった。-4℃凍結では酵素分解は停止し,非酵素的にDMAとTMAを生成した。これは-20および-40℃でも同様であった。DMAは,Fe-Cys系では-4℃より-20と-40℃で多量に生成され,微量のTMAも生成された。酵素および非酵素的DMAの生成にはいずれもFe2+と還元剤が必要であった。

A rapid screening of histamine concentration in fish fillet by direct analysis in real time mass spectrometry (DART-MS)

Daisuke Nei et 5 al.

May 1, 2017 in Food Control
Histamine is a biogenic amine causing scombroid foodborne poisoning. A rapid determination of the amine is effective in preventing the distribution of histamine-accumulated foods to market. Conventional methods for the determination of histamine concentration in foods include analyses by high performance liquid chromatography (HPLC). The present study tried to utilize a direct analysis in real-ti...

Characterization of a transglutaminase from scallop hemocyte and identification of its intracellular substrates

Hisanori Nozawa, Nobuo Seki et 3 al.

Mar 1, 2005 in Comparative Biochemistry and Physiology Part B
Scallop hemocytes contain a transglutaminase (TGase) that is electrophoretically different from the TGase in the adductor muscle. The optimum temperature of the hemocyte TGase was lower (about 15 degrees C), compared with the muscle TGase (35-40 degrees C). Other properties, such as the high sodium chloride (NaCl) and CaCl2 concentrations required for activation, instability in salt solutions, and...