The dawn of the new century has witnessed significant advances in our understanding of myocardial regeneration in both physiological and pathophysiological conditions. Several studies have divulged the existence of resident cardiac stem cells (CSCs) in heart that have the capacity to proliferate and mature into precursors which, in turn, develop into mature cardiac cell types. Various populations ...
Organoids are three-dimensional cell cultures mostly from tissue-resident or embryonic stem cells (one or multiple) on hydrogels along with defined growth factors. Currently, matrigel is the most commonly employed matrix for 3D organoid cultures. However, certain undesirable attributes of matrigel have paved the way for several other natural and synthetic hydrogel scaffolds for organoid cultures. ...
Aug 6, 2021
in International journal of molecular sciences
Runt-related transcription factor (RUNX1) regulates inflammation in non-alcoholic steatohepatitis (NASH). We performed in vivo targeted silencing of the RUNX1 gene in liver sinusoidal endothelial cells (LSECs) by using vegfr3 antibody tagged immunonano-lipocarriers encapsulated RUNX1 siRNA (RUNX1 siRNA) in murine models of methionine choline deficient (MCD) diet-induced NASH. MCD mice given nanoli...
Aug 6, 2021
in International Journal of Molecular Sciences
Background : Runt related transcription factor (RUNX1) regulates inflammation in non alcoholic steatohepatitis (NASH).
Method : We performed in vivo targeted silencing of the RUNX1 gene in liver sinusoidal endothelial cells (LSECs) by using vegfr3 antibody tagged immunonano lipocarriers encapsulated RUNX1 siRNA (RUNX1 siRNA) in murine models of methionine choline deficient (MCD) diet induced NASH...
Bioengineering, Vol. 10, Pages 131: Primary Hepatocyte Isolation and Cultures: Technical Aspects, Challenges and Advancements Bioengineering doi: 10.3390/bioengineering10020131 Authors: Impreet Kaur Ashwini Vasudevan Preety Rawal Dinesh Tripathi Seeram Ramakrishna Savneet Kaur Shiv Sarin Hepatocytes are differentiated cells that account for 80% of the hepatic volume and perform all major functions...